@article{MAKHILLJAVA201110132814, title = {Serotyping Dichelobacter nodosus with PCR-SSCP}, journal = {Journal of Animal and Veterinary Advances}, volume = {10}, number = {13}, pages = {1678-1682}, year = {2011}, issn = {1680-5593}, doi = {javaa.2011.1678.1682}, url = {https://makhillpublications.co/view-article.php?issn=1680-5593&doi=javaa.2011.1678.1682}, author = {Jon and}, keywords = {Single Strand Conformational Polymorphism (SSCP),PCR,fimA gene,Dichelobacter nodosus,sequencing,sample}, abstract = {In this study, we reported rapid approach for identification of D. nodosus serotypes that causes footrot, higly contagious diseases of the ruminants in worldwide with using Polymerase Chain Reaction-Single Strand Conformational Polymorphism (PCR-SSCP) analysis. A total of 500 swab samples from infected hoof lesion were investigated for presence of D. nodosus by PCR amplification of fimA gene. PCR-SSCP patterns revealed that each sample contained two or three different D. nodosus serotypes. Additionally on the of basis SSCP patterns, 8 different serogroups (A- G and I) were identified from New Zealand samples. These findings were confirmed by cloning and sequences of fimA gene.} }