@article{MAKHILLJAVA201312114137,
    title = {The Culture of Embryonic Stem Cells Derived from <I>in vivo</I> Fertilized Embryos of Arbas Cashmere Goats},
    journal = {Journal of Animal and Veterinary Advances},
    volume = {12},
    number = {11},
    pages = {1021-1031},
    year = {2013},
    issn = {1680-5593},
    doi = {javaa.2013.1021.1031},
    url = {https://makhillpublications.co/view-article.php?issn=1680-5593&doi=javaa.2013.1021.1031},
    author = {Dongjun,Muzi,Hui,Wenliang,Fei,Dapeng,Jianlong,Asga,Ming and},
    keywords = {Arbas Cashmere goat embryonic stem cells,passage,freezing,N2,B27},
    abstract = {Research on the embryonic stem cells of large livestock has 
  recently attracted the attention of scholars. However, it is difficult to keep 
  goat embryonic stem cells in an undifferentiated state during cell passaging 
  in culture. In this study, fertilized embryos of Arbas cashmere goats were obtained 
  by superovulation <I>in vivo</I>. The key issues in the culture of Arbas cashmere 
  Goat Embryonic Stem Cells (AgESCs) were explored: the addition of differentiation-inhibiting 
  factors, the selection of medium as well as the passaging, cryopreservation 
  and thawing methods used. This study found that high-quality <I>in vivo</I> 
  fertilized embryos could be cultured in either serum-containing medium or serum-free 
  medium and that AgESCs could be passaged in either medium for 30 generations. 
  The mechanical method was superior to the trypsin digestion method for passaging 
  AgESCs. There was no change before and after cryopreservation and thawing with 
  regard to AgESC morphology, alkaline phosphatase staining, the formation of 
  embryoid bodies, immunofluorescence staining or the PCR detection of pluripotency 
  factors. Finally this study provides the experimental basis for the establishment 
  of goat embryonic stem cell lines.}
    }