@article{MAKHILLJFT2004217846,
    title = {PCR Primers Designed from the UidA Gene Sequence for the Detection of Escherichia coli O157:H7},
    journal = {Journal of Food Technology},
    volume = {2},
    number = {1},
    pages = {59-62},
    year = {2004},
    issn = {1684-8462},
    doi = {jftech.2004.59.62},
    url = {https://makhillpublications.co/view-article.php?issn=1684-8462&doi=jftech.2004.59.62},
    author = {Moushumi Ghosh,R. Son,A. M. Sahilah,K. Sushil,W. L. Ooi,I. Salmah,S. H. Lim,Sunita Bansal and},
    keywords = {},
    abstract = {One of the uniqueness of Escherichia coli O157:H7 is it inability to ferment sorbitol in 24 h and its negative test with the MUG assay, although this organism carried the UidA gene which encodes for -glucuronidase in its chromosome. Primers were designed based on the sequence of the -glucuronidase gene and were evaluated in a polymerase chain reaction assays as a marker to detect E. coli O157:H7. Of the three pairs of primers tested, which produces the estimated product size of 352, 271 and 353 bp respectively, one primer pairs (UidA2F & UidA2R) was found to be more specific and useful as a marker in combination with a published primer for the H7 gene for the detection of Escherichia coli O157:H7.}
    }