@article{MAKHILLJEAS2019142018543,
    title = {Hypoglycemic Activity of <i>Curculigo latifolia</i> on Insulin and
Glucose Uptake Activities in in vitro},
    journal = {Journal of Engineering and Applied Sciences},
    volume = {14},
    number = {20},
    pages = {7502-7508},
    year = {2019},
    issn = {1816-949x},
    doi = {jeasci.2019.7502.7508},
    url = {https://makhillpublications.co/view-article.php?issn=1816-949x&doi=jeasci.2019.7502.7508},
    author = {Nur and},
    keywords = {Antidiabetic,antihyperglycaemic,Curculigo latifolia,glucose uptake,phenolic compounds,pancreatic cell},
    abstract = {<i>Curculigo latifolia</i> (<i>C. latifolia</i>) has been shown to potentiate the antidiabetic and hypolipidemic
effects on induced diabetic rats by altering the lipid and glucose metabolisms. In the present study, we
evaluated the effect of extracted <i>C. latifolia</i> on in vitro glucose uptake and insulin secretion by adipocyte and
investigated muscle and pancreatic cells. The aqueous extracted samples underwent polyphenol assay such
as Total Phenolic Content (TPC) and the antioxidant assay to screen the antioxidant activity of extracted
samples by inhibition of free radical from DPPH radical. With regard to in vitro, glucose uptake assay was
analysed using L6 myotube and 3T3-L1 adipocyte cell lines and insulin secretion was assayed using
BRIN-BD11 pancreatic cell in which both assays were examined under different extracted parts of samples
investigated. The results indicated that <i>C. latifolia</i> fruit possessed the highest amount of TPC
(95 mg GAE/100 g <i>C. latifolia</i> fruit extract) and antioxidant activity as it achieved the lowest IC<sub>50</sub> for DPPH assay 
(1.0&plusmn;0.1 mg/mL). Cells treated with <i>C. latifolia</i> fruit extract under insulin secretion assay has the highest
stimulation compared to others in the following descending order fruit>root>leaf. In addition, <i>C. latifolia</i> fruit
extract also showed the highest result in glucose uptake activity by 13 fold at 0.05 mg/mL with insulin presence
in 3T3-L1 adipocyte cells. However, in L6 myotube cells, <i>C. latifolia</i> fruit extract increased basal and
insulin-mediated glucose uptake activity. The results indicated the presence of antihyperglycaemic from
insulin-releasing and glucose uptake activity in <i>C. latifolia</i>.}
    }