TY  - JOUR
T1  - Detection of Ruminant-Derived Products in Animals Feed Using mtcyt-b PCR
AU - , Imadeldin E. Aradaib 
JO  - Journal of Animal and Veterinary Advances
VL  - 2
IS  - 11
SP  - 636
EP  - 638
PY  - 2003
DA  - 2001/08/19
SN  - 1680-5593
DO  - javaa.2003.636.638
UR  - https://makhillpublications.co/view-article.php?doi=javaa.2003.636.638
KW  - 
AB  - A polymerase chain reaction (PCR) assay, for detection of ruminant-derived contaminants in processed animal feed was developed. The mitochondrial cytochrome-b (mtcyt-b) gene was used as the target DNA for PCR amplification. For detection of ruminant-specific mtcyt-b gene, a nested PCR assay was used in two amplification steps. First a 735-bp product was amplified using an outer primer pair (RSL1&RSR4), then a second amplification using nested (internal) primer pairs (RSL1&RSR3) produced a 483-bp product. The sensitivity of this nested PCR-based assay was found to be 10 fg (equivalent to 1000 copies) of mtcyt-b gene, extracted from ruminant whole blood. The PCR products were easily identified by size difference following visualization on an ethidium bromide-stained agarose gel. The described nested PCR assay could be used as simple and rapid methods for detection of ruminant-derived contaminants in animal feed.
ER  -