TY - JOUR T1 - Effect of the Vitamin E on Expression of Apoptosis-Related Proteins in Immobilized Rat Testes AU - Ozan, Enver AU - Oner, Hakan AU - Kukner, Aysel AU - Oner, Jale JO - Journal of Animal and Veterinary Advances VL - 9 IS - 1 SP - 180 EP - 185 PY - 2010 DA - 2001/08/19 SN - 1680-5593 DO - javaa.2010.180.185 UR - https://makhillpublications.co/view-article.php?doi=javaa.2010.180.185 KW - spermatogonia KW -Bcl-2 KW -immobilisation KW -testes KW -Bax KW -vitamin E AB - The aim of this study was to investigate effect of the vitamin E on changes in Bax and Bcl-2 expression in the rat testes during immobilisation stress. The animals were divided into three groups. The rats in group I were immobilised for 6 h on 5 consecutive days. The rats in group II were daily injected vitamin E (100 mg kg-1) for 5 days during immobilisation. Control rats were allowed to move freely. The percentage of Bax immunpositive spermatogonia in stress group was significantly higher than those in control groups (p<0.05) but Bcl-2 was not significantly (p>0.05). The percentages of Bax and Bcl-2 immunpositive cells in the vitamin E treated group was decreased than the immobilization stress group. It was observed that Bax and Bcl-2 expression were peak level (+) in the spermatogonia of the control group. In this group, Bax and Bcl-2 expression were not determined mostly in the seminifer tubules. Neither Sertoli cells nor spermatids showed immunoreaction. Bax immunoreactivity was observed at strong level (+++) in both spermatogonia and intersititial cells of immobilised rats. In this group, Bcl-2 protein was expressed at peak level (+) in the spermatogonia. Bax expression was decreased in vitamin E treated group and at peak level (+) immunreaction was showed in the spermatogonia and intersitisyal cells. Findings of this study show that the immobilisation stress increases apoptosis and vitamin E decreases Bax and Bcl-2 expression that are apoptosis-releated proteins in the immobilised rat testes. ER -