TY - JOUR T1 - Temporal and Spatial Expression of the Pax-7 Gene During Chicken Embryo and Postnatal Development AU - Guohong, Chen AU - Teng, Ma AU - Kehua, Wang AU - Wei, Zhou AU - Ying, Zhang AU - Guobin, Chang AU - Deqin, Luan AU - Rong, Chen AU - Jing, Liao AU - Xiangping, Liu AU - Aiqing, Dai JO - Journal of Animal and Veterinary Advances VL - 10 IS - 14 SP - 1785 EP - 1788 PY - 2011 DA - 2001/08/19 SN - 1680-5593 DO - javaa.2011.1785.1788 UR - https://makhillpublications.co/view-article.php?doi=javaa.2011.1785.1788 KW - spatiotemporal expression KW -China KW -pax-7 gene KW -tissue KW -RT-PCR KW -Chicken AB - This study was conducted to investigate the different temporal and spatial expression pattern of pax-7 gene in embryo stage and growth stage of chicken, aiming to explore associations between muscle development and molecular markers of pax-7 gene. Breast and leg muscle tissue were selected from 8, 16, 20 and 21 days chick embryo and 2, 4, 6, 8, 10, 12 weeks rugao chickens. This study performed SYBR Green real-time fluorescence quantitative PCR to detect the expression pattern of pax-7 gene. The results showed that the expression pattern of pax-7 gene was similar between breast and leg muscles which it is lower during chicken embryo and reached to the highest point at 8-10 weeks of age. Pax-7 gene was continual expressed during chicken embryo and postnatal development, not only expressed in the embryo stage. Finally, we reassess current models of early patterning based on the analysis of these dynamic spatiotemporal expression patterns. ER -