TY  - JOUR
T1  - Optimization of Extraction Procedure for Chinese Truffle<I> Tuber sinense</I> Polysaccharides and Effect of the Polysaccharides on Phagocytosis of Macrophage <I>in vivo</I>
AU - Zhao, Ling AU - Wang, Kaiyu AU - Wang, Xun AU - Huang, Dan AU - Lin, Juchun AU - Chen, Defang AU - Ye, Gang AU - Lv, Cheng 
JO  - Journal of Animal and Veterinary Advances
VL  - 11
IS  - 10
SP  - 1757
EP  - 1762
PY  - 2012
DA  - 2001/08/19
SN  - 1680-5593
DO  - javaa.2012.1757.1762
UR  - https://makhillpublications.co/view-article.php?doi=javaa.2012.1757.1762
KW  - Chinese truffle Tuber sinense
KW  -polysaccharides
KW  -extraction
KW  -optimization
KW  -macrophage phagocytosis
AB  - In this study, Response Surface Methodology (RSM) was employed to optimize the extraction conditions (extraction temperature, ratio of water to raw material and extraction time) of polysaccharides from the fruiting bodies of Chinese truffle <I>Tuber sinense</I>. A Box-Behnken design was used for experimental design and analysis of the results to obtain the optimal processing parameters. The optimal conditions were extraction temperature 94.75&deg;C, ratio of water to raw material 16.41:1, extraction time 2.67 h. According to analysis, extraction temperature was the most significant factor to affect the yield of polysaccharides from Chinese truffle <I>Tuber sinense</I>. Pharmacological experiments indicated that CTP had potent immunomodulatory effects in immunosuppression mice model. At the dose of 50, 100 and 200 mg kg<SUP>-1</SUP> body weight, a significant increase (p&lt;0.01) in phagocytosis of macrophage was observed.
ER  -