TY  - JOUR
T1  - Establishment and Biological Characteristics of Hereford Cattle Fibroblast Bank
AU - Ma, Yuehui AU - Guan, Weijun AU - Zhang, Wenxiu AU - Wu, Shen AU - Li, Mei AU - Li, Changli 
JO  - Journal of Animal and Veterinary Advances
VL  - 11
IS  - 11
SP  - 1763
EP  - 1770
PY  - 2012
DA  - 2001/08/19
SN  - 1680-5593
DO  - javaa.2012.1763.1770
UR  - https://makhillpublications.co/view-article.php?doi=javaa.2012.1763.1770
KW  - livestock
KW  -fibroblast
KW  -Hereford cattle
KW  -biological characteristic
KW  -poultry
KW  -China
AB  - A fibroblast line from kindey tissue of Hereford cattle was established successfully by direct culture of explants and biology cryopreservation techniques. The cell line contained 101 tubes of frozen cells from 34 primary kidney samples. Biological analysis showed that the cells were morphologically consistent with fibroblasts and the growth curve was sigmoidal with a Population Doubling Time (PDT) of 35 h.The average viability of the cells was 95.8% before freezing and 93.4% after thawing. Cross-contamination among cell lines was excluded by isoenzyme analysis of Lactate Dehydrogenase (LDH) and Malate Dehydrogenase (MDH). The frequency of cells having the diploid chromosome number (60) was 97.5%. Detection of bacteria, fungi, viruses and mycoplasmas was verified negative. At 24, 48 and 72 h after transfection, the expression efficiency of fluorescent protein genes (<I>pEGFP-N3</I>, <I>pEYFP-N1</I> and <I>pDsRed1-N1</I>) were between 18.6~32%; The fluorescence could be observed well-distributed in cytoplasm and nucleus in addition to some cryptomere vesicles at 12 h after transfection.
ER  -