TY  - JOUR
T1  - Interactions of Target Proteins with the <I>virB4</I> Gene from Bovine Embryo Trophoblast Cells Infected with <I>Brucella abortus S</I>creened Through a Yeast Two-Hybrid Assay
AU - Chuangfu, Chen AU - Yuanzhi, Wang AU - Hui, Zhang AU - Pengyan, Wang AU - Chengyao, Li AU - Qian, Guo 
JO  - Journal of Animal and Veterinary Advances
VL  - 11
IS  - 11
SP  - 1793
EP  - 1799
PY  - 2012
DA  - 2001/08/19
SN  - 1680-5593
DO  - javaa.2012.1793.1799
UR  - https://makhillpublications.co/view-article.php?doi=javaa.2012.1793.1799
KW  - trophoblast
KW  -yeast two-hybrid assay
KW  -Brucella
KW  -virB4
KW  -embryo trophoblast cell
AB  - To screen for the interaction of target proteins with the <I>virB4</I> gene in bovine embryo trophoblast cells that were infected with Brucella bacteria. A cell culture for bovine trophoblasts was established. The cDNA library of trophoblast cells infected with the vaccine strain RB51 was constructed and a <I>Saccharomyces cerevisiae</I> expression vector containing the gene <I>virB4</I> was synthesized. Target proteins which interacted with virB4 were screened through a yeast two-hybrid system. The results showed that the cDNA library of bovine trophoblast cells infected with the vaccine RB51 strain was successfully constructed. A recombinant plasmid pGBKT7-virB4 was cloned successfully into the expression vector Y187. Thirteen different types of proteins that interacted with virB4 were screened through a yeast two-hybrid assay.
ER  -