TY  - JOUR
T1  - Effect of the Crude Polysaccharides from <I>Poria cocos</I> on mRNA Expression 
  of Adipogenic Factors During 3T3-L1 Preadipocyte Differentiation
AU - Cui, Hengmin AU - Wang, Xun AU - Liu, Haifeng AU - Zhao, Ling AU - Peng, Xi AU - Xu, Hengyong AU - Wang, Yan AU - Wang, Yuhao AU - Niang, Ling AU - Rong, Qian 
JO  - Journal of Animal and Veterinary Advances
VL  - 12
IS  - 16
SP  - 1312
EP  - 1316
PY  - 2013
DA  - 2001/08/19
SN  - 1680-5593
DO  - javaa.2013.1312.1316
UR  - https://makhillpublications.co/view-article.php?doi=javaa.2013.1312.1316
KW  - Poria cocos
KW  -polysaccharides
KW  -3T3-L1 preadipocytes
KW  -aP2
KW  -FAS
KW  -Srebf1
KW  -GLUT4
AB  - To study the effect of the crude polysaccharides from <I>Poria cocos</I> (PCCP) on mRNA expression of adipogenic factors during 3T3-L1 preadipocyte differentiation. 3T3-L1 preadipocytes were induced to differentiate with MDI (3-isobutyl-1-Methylxanthine, Dexamethasone and Insulin) induction media contained PCCP (50 &#956;g mL<SUP>-1</SUP>) and cultured for 8 days. The extent of the differentiation was assessed by Nile Red staining and GPDH activity. In addition, researchers used real-time quantitative PCR to analyzed the expression of genes related to adipocyte differentiation. The results showed that treatment of differentiating 3T3-L1 preadipocytes with PCCP (50 &#956;g mL<SUP>-1</SUP>) resulted in an increase in the expression of the adipocyte genes such as Fatty Acid System Synthase (FAS), adipocyte fatty acid binding Protein (aP2), insulin-responsive Glucose Transporter (GLUT4) and transcriptional factor genes such as CCAAT/Enhancer Binding Protein &#945; (C/EBP&#945;), peoxisome Proliferator-Activated Receptor gamma (PPAR&#947;) and Sterol Regulatory Element-Binding Protein 1c (SREBP-1c) to some degree. Overall, the data suggested that there was the tendency of increasing the expression of genes related to adipocyte differentiation in PCCP treated cells.
ER  -