TY  - JOUR
T1  - Screening for Antimicrobial Activity of Various Extracts of <I>Acanthophora spicifera</I>(Rhodomelaceae, Ceramiales) from Malaysian Waters
AU - Nor, S.A. AU - Shaida, S.F. AU - Darah, I. AU - Nurul, Z.A. 
JO  - Research Journal of Biological Sciences
VL  - 5
IS  - 5
SP  - 368
EP  - 375
PY  - 2010
DA  - 2001/08/19
SN  - 1815-8846
DO  - rjbsci.2010.368.375
UR  - https://makhillpublications.co/view-article.php?doi=rjbsci.2010.368.375
KW  - minimum bactericidal concentration
KW  -Disc diffusion method
KW  -antimicrobial activity
KW  -Acanthophora spicifera
KW  -minimum inhibitory concentration
KW  -Malaysia
AB  - The antimicrobial activities of eight crude extracts of local <I>Acanthophora spicifera </I>(Vahl) Borgesen acquired from two different extraction; soxhlet extraction and solvent partitioning were studied. These extracts were tested <I>in vitro</I> against 18 bacteria, 3 yeasts and 6 fungi strains by Disc diffusion method. The results revealed that methanol and ethyl acetate extract from solvent partitioning exhibited broader spectrum activity against tested bacterial strains. This two extracts showed inhibition zones against strains of <I>Bacillus cereus</I> ATCC 10876, <I>Bacillus licheniformis</I> ATCC 12759, Menthicilin Resistance<I> Staphyloccocus aureus </I>(MRSA), <I>Pseudomonas aeruginosa</I> ATCC 27853, <I>Yersinia</I> sp. and <I>Citrobacter freundii</I>. While methanol extracts from Soxhlet extraction and butanol from solvent partitioning had no effect against <I>P. aeruginosa</I> ATCC 27853, the other six extracts exhibited antibacterial activity against this opportunistic strain. Extracts showed a moderate average zone of inhibition ranged from 9.00-14.00 mm. <I>Yersinia</I> sp. showed the higher inhibition zones of 12.00 mm. However, no antifungal activity observed against tested strains of yeasts and fungi. The MIC and MBC values of selected bacterial strains tested ranged from 31.25-1000 &#956;g mL<SUP>-1</SUP> and 500-2000 &#956;g mL<SUP>-1</SUP>.
ER  -