TY  - JOUR
T1  - Evaluation of the Two-Component Regulatory System Genes in <I>Staphylococcus aureus</I> with Roles for Survival and Pathogenicity
AU - Sekawi, Zamberi AU - Nik Ibrahim, Nik Khairul Azizi AU - Shamsudin, Mariana Nor 
JO  - Research Journal of Biological Sciences
VL  - 5
IS  - 11
SP  - 731
EP  - 734
PY  - 2010
DA  - 2001/08/19
SN  - 1815-8846
DO  - rjbsci.2010.731.734
UR  - https://makhillpublications.co/view-article.php?doi=rjbsci.2010.731.734
KW  - reserve transcriptase
KW  -TCRS
KW  -MRSA
KW  -antibiotics
KW  -regulatory gene
KW  -Staphylococcus aureus
AB  - The current research trend for a new drug development was expanded by using the proteomic approaches. However, the expression on those drug target must be well identified before taking part into proteomics study. One of the most commonly use protocol is the expression profiles on those selected genes during certain simulated conditions. <I>Staphylococcus aureus</I> has been blessed with several virulence factors which are essential for its pathogenicity. One of the most well studied systems is the Two-Component Regulatory Systems (TCRSs). The TCRSs play a vital role in <I>S. aureus</I> survival and it become a good candidate for the specific drug target. Prior to determining the role, this study was made to observe the expression pattern of two TCRS’ regulatory genes during the treatment with antibiotics against two strains of <I>S. aureus</I>; the Methicillin Methicillin Susceptible <I>S. aureus</I> (MSSA) and Methicillin Resistant <I>S. aureus</I> (MRSA). The partial transcripted analysis of the Reverse Transcriptase PCR (RT-PCR) data shows that the rates of expression in selected <I>TCRS</I> genes were different in both strains. Knowing the importance to <I>S. aureus</I> growth and pathogenesis, the <I>TCRS </I> genes can be a good candidate for designing a novel specific drug target against <I>S. aureus</I>, especially the MRSA.
ER  -