TY  - JOUR
T1  - Inhibition Kinetics of Phenol Degradation by Binary Mixed Culture from Continuous Culture and Wash-Out Data
AU - , S.E. Agarry AU - , E. Betiku AU - , B.O. Solomon 
JO  - Journal of Engineering and Applied Sciences
VL  - 2
IS  - 6
SP  - 1020
EP  - 1026
PY  - 2007
DA  - 2001/08/19
SN  - 1816-949x
DO  - jeasci.2007.1020.1026
UR  - https://makhillpublications.co/view-article.php?doi=jeasci.2007.1020.1026
KW  - Continuous cultivation
KW  -washout cultivation
KW  -steady state
KW  -substrate inhibition
KW  -bioreactor
KW  -primary culture
KW  -secondary culture
KW  -kinetic parameters
KW  -mixed culture
AB  - Steady states of a continuous culture with an inhibitory substrate was used to estimate kinetic parameters under substrate limitation (chemo stat operation) Mixed cultures of an indigenous <I>Pseudomonas fluorescence </I>and<I> Pseudomonas aeruginosa</I> were grown in continuous culture on phenol as the sole source of carbon and energy at dilution rates of 0.010-0.20 h <SUP>1</SUP>. Using different dilution rates several steady states were investigated and the specific phenol consumption rates were calculated. In addition, phenol degradation was investigated by increasing the dilution rate above the critical dilution rate (washout cultivation). The results showed that the phenol degradation by mixed culture of <I>Pseudomonas fluorescence </I>and<I> Pseudomonas aeruginosa</I> can be described by simple substrate inhibition kinetics under substrate limitation but cannot be described by simple substrate inhibition kinetics under washout cultivation. The phenol consumption rate (degradation rate) increased with increase in dilution rate. Fitting of the steady state data from continuous cultivation to various inhibition models resulted in the best fit for the Teissier kinetic inhibition model. The r<SUB>smax </SUB>value of 0.322 mg/mg/hr obtained from the Teissier equation was comparable to the experimentally calculated r<SUB>smax</SUB> value of 0.342 mg/mg/hr obtained under washout cultivation.
ER  -