TY  - JOUR
T1  - Characterization of Lignocellulosic Enzymes from White-rot Fungus <I>Phlebia chrysocreas</I> Isolated from a Marine Habitat
AU - , Godliving Mtui AU - , Yoshitoshi Nakamura 
JO  - Journal of Engineering and Applied Sciences
VL  - 2
IS  - 10
SP  - 1501
EP  - 1508
PY  - 2007
DA  - 2001/08/19
SN  - 1816-949x
DO  - jeasci.2007.1501.1508
UR  - https://makhillpublications.co/view-article.php?doi=jeasci.2007.1501.1508
KW  - Lignocellulosic enzymes
KW  -biodegradation
KW  -gel filtration chromatography
KW  -electrophoresis
KW  -isoelectric focusing
AB  - Marine fungus <I>Phlebia chrysocreas </I>was isolated from decomposing mangrove leaves in Western Indian Ocean cost and cultured in the laboratory. Protein content and lignocellulosic enzyme activities were measured by photometric methods. Desalted and size-separated enzyme filtrates were resolved by sodium Docecyl Sulphate-Polyacrylamide Gel Electrophoresis (SDS-PAGE) and Isoelectric Focusing (IEF). The fungal filtrate  had  maximum  Lignin  Peroxidase  (LiP),  Manganese  Peroxidase (MnP) and Laccase (Lac), activities of 45, 37 and 11 U mL <SUP>1</SUP>, respectively. <I>P. chrysocreas</I>, showed effective (92-100%) decolorization of synthetic azo dyes (Azure-B, Poly-B and Poly-R) in liquid cultures and completely decolorized textile wastewater in immobilized cultures. The LiP and MnP from <I>P. chrysocreas</I> purified by gel filtration chromatography revealed it to have molecular weights of 46 kDa and 47 kDa and isoelectric points of 4.0 and 3.8, respectively. Purified fractions had optimal reaction rates at temperature of 30ºC for both LiP and MnP while optimal pH for LiP and MnP were 4.5 and 5.0, respectively. The study confirmed extracellular enzymes from<I> P. chrysocreas</I> to be potential degraders of organic pollutants and showed that facultative marine fungi that live under harsh seawater conditions are suitable for bioremediation of recalcitrant compounds.
ER  -