TY  - JOUR
T1  - Detection and Cloning of Alpha Glucosidase Inhibitor Gene
<I>Streptomyces</I> sp. IPBCC. B. 15.1539 and Potential as an
Anti Hyperglycemic in Education Science
AU - Makbuloh, Deden AU - Velina, Yessy 
JO  - Journal of Engineering and Applied Sciences
VL  - 12
IS  - 8
SP  - 1987
EP  - 1991
PY  - 2017
DA  - 2001/08/19
SN  - 1816-949x
DO  - jeasci.2017.1987.1991
UR  - https://makhillpublications.co/view-article.php?doi=jeasci.2017.1987.1991
KW  - Diabetes mellitus
KW  -Streptomyces sp. IPBCC. B. 15.1539
KW  -inhibitor "-glucosidase sedoheptulose 7-phosphate cyclase
KW  -nucleotide
KW  -aminocyclitols
AB  - Diabetes mellitus is a metabolic disorder characterized by the presence of hyperglycemia due to
defective insulin secretion, defective insulin action or both. One therapeutic approach for treating diabetes is
to decrease the post-prandial hyperglycemia. This is done by preventing the absorption of glucose through
the inhibition of the carbohydrate-hydrolysing enzymes &alpha;-glucosidase and &alpha;-amylase in the digestive tract.
Actinomycetes have been known as source of commercialized acarbose, an &alpha;-glucosidase inhibitor. Acarbose
is keto analog moieties of the C7N aminocyclitols. Sedoheptulose 7-phosphate is converted into
2-5 epi-valiolone via the activity of sedoheptulose 7-phosphate cyclase at the first step of the biosynthesis of
C7N aminocyclitol. This research was aimed to detect and cloning sedoheptulose 7-phosphate cyclase gene.
Detection gene was done by using Polymerase Chain Reaction (PCR) with with design primers C7N
aminocyclitol. The primer used was designed on the basis of the comparison of the sequence of known
sedoheptulose 7-phosphate cyclase (ACBC) from that of <I>Actinoplanes</I> sp. SE50/100 which was then cloned
by T-Vector pMD20. The result show that there was similiarity of nucleotide series sedoheptulose 7-phosphate
cyclase of Streptomyces sp. IPBCC. B. 15.1539 with GenBank database analysis through blast program. The
result indicated that the designed primer was able to amplify the sedoheptulose 7-phosphate cyclase acarbose
although the accomplishment in amplifying the gene is still up to 300 bp and have 100% sequence similiarity
with DNA fragment of sedoheptulose 7-phosphate cyclase <I>Actinoplanes</I> sp. complete acarbose (ACB) gene
cluster, strain SE50/110, accses number Y18523.4 reported in GenBank.
ER  -