TY - JOUR T1 - Optimization of Hydrogen Production Process by Response Surface Methodology AU - Saifuddin, N. AU - Priatharsini, P. AU - Ong, M.Y. JO - Journal of Engineering and Applied Sciences VL - 12 IS - 19 SP - 5050 EP - 5057 PY - 2017 DA - 2001/08/19 SN - 1816-949x DO - jeasci.2017.5050.5057 UR - https://makhillpublications.co/view-article.php?doi=jeasci.2017.5050.5057 KW - Bio-hydrogen KW -Response Surface Methodology (RSM) KW -optimization KW -microalgae KW -ragi tapai KW -amount AB - Enhancement of bio-hydrogen production by green microalgae Chlamydomonas reinhardtii has captivated tremendous interest. The yield of bio-hydrogen production by C. reinhardtii was enhanced by co-culturing with ragi tapai followed by immobilization and culturing in the right TAP-S medium. In this study, the three factors that affects the bio-hydrogen production by C. reinhardtii were evaluated with Response Surface Methodology (RSM). The amount of microalgae and ragi tapai as well the pH of the culture medium were simultaneously altered to determine the maximum bio-hydrogen yield. The maximum bio-hydrogen production yield obtained was 702317 ppm under conditions of 2.46 g of microalgae, 0.45 g of ragi tapai and pH medium of 7.23. ER -