TY  - JOUR
T1  - The Analysis of Bacterial Diversity in High Thermic-Low Salinity Oil
Reservoirs Prior to Microbial Enhanced Oil Recovery Field Test
AU - Adhitya Purwasena, Isty AU - Sugai, Yuichi 
JO  - Journal of Engineering and Applied Sciences
VL  - 13
IS  - 11
SP  - 4104
EP  - 4112
PY  - 2018
DA  - 2001/08/19
SN  - 1816-949x
DO  - jeasci.2018.4104.4112
UR  - https://makhillpublications.co/view-article.php?doi=jeasci.2018.4104.4112
KW  - screening
KW  -bacterial diversity
KW  -oilfield
KW  -DGGE
KW  -MEOR
KW  -16S rDNA
AB  - Nutrition injection in Microbial Enhanced Oil Recovery (MEOR) process might stimulate not only
desirable microorganisms but also the undesirable ones. Therefore, studying the properties of bacterial
community in reservoir prior to field test is a critical step in MEOR process. In this study, bacterial communities
from high thermic and low salinity reservoirs located in Japan, China and Indonesia were characterized by 16S
rDNA sequence analysis. Bacterial genomic DNAs were extracted from produced water samples and their
sequences were evaluated by genetic fingerprinting based approach. PCR-DGGE analyses showed multiple
bands in all produced water samples which indicated high bacterial diversities. Sequences identified in this
study were mainly related to only five phyla, i.e., Bacteroidetes, Thermotogae, Defferibacteres, Firmicutes
and Proteobacteria, suggesting that phylotype richness is low in oil reservoirs. One sequence of
Bacteriodetes-affiliated bacteria which showed 99% similarity with Bacteriodales bacterium 5bM was retrieved
from DGGE results of all oilfields. This result showed that, Bacteriodetes can grow well in Indonesia, Japan and
China oilfield, making it the best bacterial candidate for MEOR field test. Results showed in this study revealed
that finger printing is a considerable technique for microbial screening prior to MEOR field test application.
ER  -