TY  - JOUR
T1  - Detection of &beta;-Lactam Resistance PER-1 Gene in Multidrug Resistant Isolates of <i>Acinetobacter baumannii</i> Isolated from Urinary Tract Infections in Diyala City
AU - Jasim, Izdehar M. 
JO  - Journal of Engineering and Applied Sciences
VL  - 14
IS  - 13
SP  - 4448
EP  - 4452
PY  - 2019
DA  - 2001/08/19
SN  - 1816-949x
DO  - jeasci.2019.4448.4452
UR  - https://makhillpublications.co/view-article.php?doi=jeasci.2019.4448.4452
KW  - PER-1 gene
KW  -multidrug resistant
KW  -Acinetobacter baumannii
KW  -antibiotics
KW  -technique
KW  -culture
AB  - The detection of &beta;-lactam-resistant<i> A. cinetobacter baumannii</i> in Diyala city and their resistance
genetic mechanisms was undertaken. We studied the extended-spectrum beta-lactamase genes, particularly the
PER-1 gene, among &beta;-lactam-resistant <i>A. baumannii</i> isolates from patients from patients suffering from urinary
tract infections in teaching Baquba hospital and Al-Betul hospital in Diyala city. From April to October (2015),
250 urine samples were collected. And the results of bacterial culture on media of MacConkey agar, blood agar
and biochemical tests and confirm the diagnosis using Api 20 E system and Vitek 2 compact system showed
that 8 isolates are belonging to bacteria of <i>Acinetobacte baumannii</i>. Fresh subculture samples were tested for
antimicrobial susceptibility against (11) antibiotics using disk diffusion method, the results revealed that all
isolates were variable resistance to ampicillin, aztreonam, ceftazidium, cefotaxime, cefepime, ciprofloxacin,
piperacillin, meropenem, nalidixic acid and amikacin (62.5, 50, 75, 75, 50, 50, 75, 37.5, 50 and 25%), respectively.
Whearase all isolates were sensitive to antibiotic imipenem. Total genomic DNA was extracted from each isolate
and further used for Polymerase Chain Reaction (PCR). The results of detection of &beta;-lactam resistance gene by
molcular technique PCR revealed that of these 8 isolates, 6 were found to harboring PER-1 gene.
ER  -