TY  - JOUR
T1  - Chicken Feather Hydrolysate as Potential Peptone Source for
Bacterial and Yeast Cultivation
AU - B. Akpor, Oghenerobor AU - M. Oluba, Olarewaju AU - Alabi, Olayinka O. AU - G. Adeyonu, Abigail AU - J. Shoyomb, Ayoola 
JO  - Journal of Engineering and Applied Sciences
VL  - 14
IS  - 23
SP  - 8721
EP  - 8726
PY  - 2019
DA  - 2001/08/19
SN  - 1816-949x
DO  - jeasci.2019.8721.8726
UR  - https://makhillpublications.co/view-article.php?doi=jeasci.2019.8721.8726
KW  - Microbial cultivation
KW  -feather hydrolysate
KW  -growth rate
KW  -microorganisms
KW  -isolates
KW  -species
AB  - To reduce the production cost of growth medium for microbial cultivation, a variety of natural
products such as milk, animal tissues and plants have been exploited. The present study was aimed at
generating Chicken Feather Protein Hydrolysate (CFPH) from alkaline hydrolysis of chicken feathers as a
medium component in the cultivation of selected bacteria and yeasts. Alkaline hydrolysis of the raw chicken
feather was carried out to obtain CFPH. The study was carried out using different combinations of CFPH:
peptone ratios for the growth medium. Three bacteria <em>Esherichia coli, Staphylococcus aureus</em> and <em>Klebsiella
pneumonia</em>) and two yeasts (<em>Candida carpophila</em> and <em>Candida tropicalis</em>) species were as the test microbial
species used as test microorganisms. For growth rate studies, 0.5 mL of the broth cultures of the respective test
isolates was inoculated in the growth medium. Immediately after inoculation and at every 2 h interval, for
a 10 h duration, aliquot samples of the inoculated broth cultures were aseptically withdrawn from each flask for
optical density measurement. The study revealed 60 % CFPH yield with the hydrolysis method employed. The
results obtained demonstrated that growth performance of the test microorganisms varied for each of the CFPH:
peptone combinations. Generally, growth rates of the yeast were observed to be significantly higher (p<0.05)
in media with CFPH: peptone combinations of 4:6, 6:4 and 8:2 than media with peptone only. Although, in most
of the bacteria species investigated, growth was better in the peptone only media, media containing 4:6, 6:4 and
8:2 CFPH: peptone combinations compared well in terms of growth rate. Data obtained in this study showed
the potential of CFPH as an alternative growth substrate to peptone in microbial culturing. Thus, revealing the
possibility of conversion of chicken feather waste to more valuable use.
ER  -