TY  - JOUR
T1  - Effect of Isoelectric Precipitation and Ultrafiltration Treatment on Extraction and
Physicochemical Properties of Proteins from Date Palm Pollen
AU - Sebii, Haifa AU - Karra, Sirine AU - Gafsi Makhlouf, Ines AU - Danthine, Sabine AU - Blecker, Christophe AU - Attia, Hamadi AU - Besbes, Souhail AU - Bchir, Brahim 
JO  - Journal of Engineering and Applied Sciences
VL  - 16
IS  - 2
SP  - 103
EP  - 110
PY  - 2021
DA  - 2001/08/19
SN  - 1816-949x
DO  - jeasci.2021.103.110
UR  - https://makhillpublications.co/view-article.php?doi=jeasci.2021.103.110
KW  - Date palm pollen
KW  -ultrafiltration
KW  -isoelectric precipitation
KW  -protein concentrate
KW  -surface properties
KW  -thermal properties
AB  - The present study investigates a comparison of
two methods of extraction of date palm pollen protein.
Two obtained pollen protein concentrates using the
isoelectric precipitation and the ultrafiltration methods,
(PPCIP and PPCUF) were obtained. From a physical
point of view, PPCIP was a crystalline powder whereas
PPCUF had an amorphous structure. The surface
properties of the two protein concentrates varied
according to pH and concentration. Indeed, it turned out
that at pH 7, the two extracts were more capable of
reducing the surface tension than at pH 4. Findings also
proved that the surfactant proteins had a molecular mass
>10 KDa. In addition, the solubility values were greater
at pH 7 than at pH 4. These results were in line with the
isoelectric points determined in the ZETA potential study
which gave a pHi around 3 for the two extracts.The
differential calorimetric analysis revealed that PPCIP had
two denaturation peaks that could be attributed to the
different constituents contained in the extract whereas
PPCUF presented only one peak relative to the
denaturation of DPP proteins. This difference was
simultaneously noticed in the Thermogravimetric
analysis.
ER  -