TY - JOUR
T1 - Comparative Assessment of Stabilizers Used for Freeze-Drying T1/44 Contagious Bovine Pleuropneumonia Vaccine (CBPPV) Produce in Nigeria
AU - , E.J. Ngbede
JO - Research Journal of Animal Sciences
VL - 1
IS - 3
SP - 85
EP - 88
PY - 2007
DA - 2001/08/19
SN - 1993-5269
DO - rjnasci.2007.85.88
UR - https://makhillpublications.co/view-article.php?doi=rjnasci.2007.85.88
KW - Stabilizer
KW -freeze-dried
KW -Contagious Bovine Pleuro-Pneumonia Vaccine (CBPPV)
KW -assessment of stabilizers
AB - The effect of using 85% buffered sucrose solution plus gelatin ("S") as stabilizer and a combination of buffered sucrose/lactalbuminhydrolysate solution in ratio 20:5% (w/w), respectively with gelatin ("L") as stabilizer during freeze-drying process and under varying storage temperature conditions on CBPP vaccine was investigated. It was found that both stabilizers gave good freeze-dried preparation of T1/44 CBPP vaccines. During the process of freeze-drying, there was a total average loss of 4.73 cfu mL 1viable organisms in vaccine with stabilizing medium "L" plus gelatin and an average loss of 5.25 cfu mL 1 viable organisms for vaccine with stabilizing medium "S" plus gelatin. The results favoured the choice of sucrose/lactalbuminhydrolysate plus gelatin stabilizer on viability of T1/44 Mycoplasma mycoides subsp. mycoides during freeze-drying process. Vaccine with stabilizing medium "L" plus gelatin stored at +4°C for 15 months maintained sufficient viable mycoplasma to protect cattle. The vaccine titre dropped from 1.5×109-4.3×107 cfu mL 1, while vaccine with stabilizing medium "S" plus gelatin for the same period dropped from 3.1×109-1.8×107 cfu mL 1. At room temperature of 25°C, the 2 types of vaccines maintained sufficient viable mycoplasma up to 4 months, but detrimental as the titre may drop suddenly when reconstituted for vaccination in the field. Storage of this vaccine at 37°C is not recommended for the same reason stated above.
ER -