TY  - JOUR
T1  - Production of Indole-3-Acetic Acid by <I>Rhizobium </I>Isolates from <I>Sesbania</I> Species
AU - , M. Sridevi AU - , K.V. Mallaiah 
JO  - Plant Sciences Research
VL  - 1
IS  - 1
SP  - 13
EP  - 16
PY  - 2008
DA  - 2001/08/19
SN  - 1995-476x
DO  - psres.2008.13.16
UR  - https://makhillpublications.co/view-article.php?doi=psres.2008.13.16
KW  - Rhizobium sp.
KW  -indole acetic acid
KW  -Sesbania sp.
KW  -rhizobium-legume symbiosis
AB  - <I>Rhizobium</I> strains isolated  from  root  (<I>Sesbania  procumbens</I>)  and stem nodules (<I>S. rostrata</I> and <I>S. procumbens</I>) of <I>Sesbania</I> species were tested for their ability to produce Indole-3-Acetic Acid (IAA). The isolates produced maximum amount of IAA in culture after 72 h of incubation and at 3.0 mg mL <SUP>1</SUP> L-tryptophan concentration. The effect of different carbon and nitrogen sources on IAA production was also studied and it revealed that, glucose (1%) and potassium nitrate (0.1%) were found to be best promoters for IAA production. An addition of EDTA (0.1 µg mL <SUP>1</SUP>) significantly increased the IAA production over controls and the production was maximum at 0.2 µg mL <SUP>1</SUP> EDTA concentration. Among the three isolates, maximum amount of IAA was produced by <I>Rhizobium</I> isolate from stem nodule of <I>S. procumbens</I>. The IAA was extracted, purified and identified by thin layer chromatography.
ER  -