TY - JOUR
T1 - Isolation, Similarity and Subcellular Localisation of Transaldolase from
Sugarcane (Saccharum officinarum)
AU - Nulit, R. AU - Kalhori, Nahid AU - Go, Rusea
JO - Journal of Molecular Genetics
VL - 5
IS - 1
SP - 1
EP - 2
PY - 2013
DA - 2001/08/19
SN - 2070-4267
DO - jmolgene.2013.1.2
UR - https://makhillpublications.co/view-article.php?doi=jmolgene.2013.1.2
KW - The oxidative pentose pathway
KW -transaldolase
KW -subcellular localisation
KW -E4P
KW -TAL gene
AB - This study focused on isolation, cloning of TAL from sugarcane.
Transaldolase is one of the enzymes of the Pentose Phosphate Pathway (PPP).
Transaldolase in non-oxidative phase of OPPP transfer a three carbon dihydroxyacetone
moiety from sedoheptulose-7-phosphate and glyceraldehyde-3-phophate to produce
Erythrose-4-Phophate (E4P) and fructose-6-phophate. E4P is the precursor for
many secondary metabolic pathways including aromatic amino acids, lignin and
flavonoid synthesis. Earlier studies revealed that OPPP is incomplete in the
cytosol of plants as no genes encoding for a cytosolic TAL. Moreover, there
is no study about the TAL genes from sugarcane until to date. Thus, the
objective of this study is to isolate TAL gene from sugarcane, to compare
its similarity with other plants, to determine its subcellular localization.
A total of 1601 bp of TAL has been isolated by PCR. Similarity, studies by ClustalW
revealed that TAL show highest similarity (75%) with Zea mays. Analysis
of subcellular localization by using Target 1.1 revealed that of TAL from sugarcane
was not located in the plastidic.
ER -