Marek Kmiec  , Daniel Polasik  , Filip Napierala  , Iwona Polasik  , 
    GH/HaeII and GH/MspI Restriction Polymorphism in a Herd of Polish Large White Sows,
    Journal of Animal and Veterinary Advances,
    Volume 7,Issue 2,
    2008,
    Pages 165-169,
    ISSN 1680-5593,
    javaa.2008.165.169,
    (https://makhillpublications.co/view-article.php?doi=javaa.2008.165.169)
    Abstract: The study covered a herd of 300 polish large white sows kept in one pigsty in the West-Pomeranian Region in Poland. The polymorphism of a selected 506-bp GH gene fragment was detected by the PCR-RFLP method using specific primer sequences and endonuclease HaeII and MspI. In the studied herd of sows, there were found to be three GH/HaeII genotypes (h<SUP>-</SUP>h<SUP>-</SUP>-4.7%, h<SUP>+</SUP>h<SUP>-</SUP>-54% and h<SUP>+</SUP>h<SUP>+</SUP>-41.3%) and three GH/MspI genotypes (m<SUP>-</SUP>m<SUP>-</SUP> 30%, m<SUP>+</SUP>m<SUP>-</SUP>-23.3% and m<SUP>+</SUP>m<SUP>+</SUP>-46.7%), each of them determined by two alleles. The GH/HaeII allele frequencies were 31.7% for allele h<SUP>- </SUP>and 68.3% for allele h<SUP>+</SUP>, whereas the GH/MspI allele frequencies were 41.7% for allele m<SUP>- </SUP>and 58.3% for allele m<SUP>+</SUP>. The genetic equilibrium in the herd under study was found to be disrupted as the number of individuals observed in the GH/HaeII and GH/MspI genotype groups was significantly different from their expected number calculated according to the Hardy-Weinberg law. The study proved the existence of GH/HaeII and GH/MspI polymorphisms in a selected GH gene sequence in Polish Large White sows and revealed an association between the GH/HaeII and GH/MspI genotypes and reproductive traits of the sows.
    Keywords: Growth hormone gene;GH;polymorphism;reproductive traits;polish large white sows