Bang  Liu, Ling  Sun, Xinjie  Dong, Bin  Fan, 
    The Association of ANKRD2 with Loin Depth and Muscle Firmness in Pigs,
    Journal of Animal and Veterinary Advances,
    Volume 10,Issue 11,
    2011,
    Pages 1462-1468,
    ISSN 1680-5593,
    javaa.2011.1462.1468,
    (https://makhillpublications.co/view-article.php?doi=javaa.2011.1462.1468)
    Abstract: The Muscle Ankyrin Repeat Proteins (MARPs) family is essential for cardiogenesis and Cardiac hypertrophy (CARP), skeletal muscular stretch and development (ANKRD2/ARPP) and during recovery following starvation (DARP). Here we describe the isolation and molecular characterization of the full-length cDNA of porcine gene <I>ANKRD2 </I>(ankyrin repeated domain 2). The cDNA sequence and predicted protein sequence shared high sequence identity with other mammalian counterparts. The Reverse Transcriptase-Polymerase Chain Reaction (RT-PCR) revealed that ANKRD2 has a high level of expression in skeletal muscle and a low expression in heart while the other tissues being tested had no expression. The expression of <I>ANKRD2</I> gene was up-regulated accompanying with the different embryo developmental stages (embryo 33, 65, 90 days; postnatal 2 days, adult) and reached the peak at the adult time. A T/C single nucleotide polymorphism in ANKRD2 exon5 was detected and a HaeIII PCR-Restriction Fragment Length Polymorphism (PCR-RFLP) was then developed. Association of the genotypes with growth and carcass traits revealed significant associations of the porcine ANKRD2 loin depth (p&lt;0.05) and firmness (p&lt;0.05).
    Keywords: ANKRD2;meat quality trait;expression profile;association analysis;Sequence analysis;carcass trait