Lih-Ren  Chen, Jen-Wen  Shiau, Chein  Tai, Jenn-Fa  Liou, 
    Production of Egg Yolk Immunoglobulin Against <I>Escherichia coli</I> From White Leghorn and Lohmann Chickens,
    Journal of Animal and Veterinary Advances,
    Volume 10,Issue 18,
    2011,
    Pages 2349-2356,
    ISSN 1680-5593,
    javaa.2011.2349.2356,
    (https://makhillpublications.co/view-article.php?doi=javaa.2011.2349.2356)
    Abstract: This study investigates the immunization response of different 
  breeds of chickens to<I> Escherichia coli</I> (<I>E. coli</I>) antigen and the 
  function of specific Immunoglobulin in Yolk (IgY) produced. The antibody was 
  raised by the intramuscular immunization of White Leghorn hens and Lohmann hens 
  with inactivated <I>E. coli </I>antigen either from a Commercial vaccine (CM) 
  or isolated from a local farm (AF). Anti-<I>E. coli </I>IgY antibodies were 
  isolated using the water extraction method and their <I>E. coli</I> inhibitory 
  effects were then determined. The anti-<I>E. coli</I> IgY production levels 
  of White Leghorn chickens/Lohmann chickens immunized with the CM and AF antigens 
  were 65.28/55.78 &#956;g mL&oline;<SUP>1</SUP> WSF and 48.31/14.4 &#956;g mL&oline;<SUP>1</SUP> 
  WSF, respectively. The specific IgY from White Leghorn chickens immunized with 
  CM antigen exhibited a superior inhibitory effect on the growth of activated 
  <I>E. coli</I>. The least effective concentration to inhibit the growth of activate 
  <I>E. coli</I> for this specific IgY was 63 &#956;g mL&oline;<SUP>1</SUP> WSF. 
  The passive protective effect of egg-yolk antibodies against <I>E. coli </I>K99 
  infection in neonatal piglets indicates that piglets treated with antibodies 
  from the hens immunized against Enterotoxigenic<I> E. coli</I> (ETEC) were protected 
  against the deleterious effects of this organism.
    Keywords: IgY;E. coli;antibodies;egg-yolk;immunized;chicken