Qing-Hui  Jia, Zhi-Xin  Fu, Chen  Juan, Zheng-Zhu  Liu, Shen  Ping, Li-Xin  DU, Chuan-Sheng  Zhang, Xie-Rong  Liu, Li-Ying  Geng, 
    Culture of Chicken Embryonic Stem Cells in Buffalo Rat Liver 3A Cells (BRL-3A) Conditioned Medium,
    Journal of Animal and Veterinary Advances,
    Volume 10,Issue 6,
    2011,
    Pages 791-795,
    ISSN 1680-5593,
    javaa.2011.791.795,
    (https://makhillpublications.co/view-article.php?doi=javaa.2011.791.795)
    Abstract: BRL-3A conditioned medium combined with basic Fibroblast Growth Factor (bFGF), Insulin-like Growth Factor (IGF), Stem Cell Factor (SCF) and STO cells are used for the culture of the feeder layer and ES (Embryonic Stem) cell clones. This study is done by isolation of chicken X-stage blastodermal cells. The putative ES cell clone features a typical bird&#146;s nest structure, PAS and AKP-positive and strongly positive for SSEA-1. It is able to form embryoid bodies and multiple types of cells as well as participates in embryonic development and feather color chimera formation through <I>in vitro</I> differentiation.
    Keywords: cell clones;BRL-3A cells;in vitro culture;ES cell;Chicken;embryonic stem