D.K. Chen  , C.C.  Shang, Y.Q. Zhao  , D.Y. Cao  , Y.L. Yao  , J. Luo  , 
    Cloning and Expression of Transforming Growth Factor Beta 1 of Guanzhong Dairy Goat and its Effect on Proliferation of Goat Peripheral Blood Mononuclear Cells (PBMCs),
    Journal of Animal and Veterinary Advances,
    Volume 11,Issue 8,
    2012,
    Pages 1127-1134,
    ISSN 1680-5593,
    javaa.2012.1127.1134,
    (https://makhillpublications.co/view-article.php?doi=javaa.2012.1127.1134)
    Abstract: Transforming Growth Factor beta 1 (TGF-&#946;1) is a pleiotropic cytokine that affects diverse biologic processes. In this study, the goat <I>TGF-&#946;1</I> gene was cloned and sequenced with length of 1173 bp. The predicted peptide of goat TGF-&#946;1 contained 390 amino acids and 29 amino acids signal peptide. Phylogenetic analysis of different species available in GenBank<SUP>TM</SUP> based on the complete amino acid sequences of TGF-&#946;1 showed that the goat was clustered with sheep sistered to bovine. The TGF-&#946;1 mature peptide including 112 amino acids was expressed with a fusion protein of approximately 32 kDa. The goat PBMCs were proliferative stimulated by recombinant TGF-&#946;1 but the proliferation of PBMCs stimulated by ConA was inhibited under co-stimulation of recombinant TGF-&#946;1. These results suggest that the goat recombinant TGF-&#946;1 would play an important role in regulating the immune responses and sustaining the balance of organism physiological state.
    Keywords: TGF-B1;PBMCs;proliferation;dairy goat;physiological state;amino acid