Li  Shou-Jun, Wang  Li, Guo  Yu-Fei, Qi  Hai-Tao, Su  Shuo, Chen  Ji-Dang, Zhao  Fu-Rong, Cao  Nan, Zhang  Min-Ze, Zhang  Chao-Yi, Huang  Zhen, Tan  Li-Kai, Zhang  Gui-Hong, 
    Amplification of Nucleoprotein (<I>NP</I>) Gene from Canine Influenza Virus 
  H3N2 and its Expression in <I>Escherichia coli</I>,
    Journal of Animal and Veterinary Advances,
    Volume 11,Issue 14,
    2012,
    Pages 2432-2435,
    ISSN 1680-5593,
    javaa.2012.2432.2435,
    (https://makhillpublications.co/view-article.php?doi=javaa.2012.2432.2435)
    Abstract: NP is a relatively conservative structure protein also is the main recognition antigen and have good middle cross reactive. To express Canine Influenza Virus (CIV) subtype H3N2 NP protein, the <I>NP</I> gene was amplified by PCR and cloned into vector pMD18-T and then sequence determination. The <I>NP</I> gene was cloned into vector pET-32a (+). The recombinant plasmid pET-NP was transformed into Rosetta II (DE3) and induced with IPTG.
    Keywords: Canine influenza virus;NP gene;cloning;gene expression;protein