Umar Farooq Awan  , Shazia Mirza  , Kiran Shafiq  , Sikander Ali  , Ikram-ul-Haq  , 
    Biosynthesis of Lipase by Rhizopus oligosporous ISUUV-16 using Agricultural By-products as Substrate,
    Journal of Food Technology,
    Volume 1,Issue 3,
    2003,
    Pages 111-114,
    ISSN 1684-8462,
    jftech.2003.111.114,
    (https://makhillpublications.co/view-article.php?doi=jftech.2003.111.114)
    Abstract: The present study is concerned with the biosynthesis of lipase by Rhizopus oligosporous ISUUV-16 through solid-state fermentation. The optimal substrate for the biosynthesis of lipase was almond meal at the level of 10 g per 250 ml Erlenmeyer flask. The optimal lipase production was obtained when incubated at 300C, for 48 h. Maximal lipase activity of 54 Ug-1 was reached at 1 % level of inoculum. Water was used as diluent for enzyme extraction from fermented mash. Substrate to diluent ratio was kept at 1:0.7.
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