E.J. Ngbede  , 
    Comparative Assessment of Stabilizers Used for Freeze-Drying T<SUB>1</SUB>/44 Contagious Bovine Pleuropneumonia Vaccine (CBPPV) Produce in Nigeria,
    Research Journal of Animal Sciences,
    Volume 1,Issue 3,
    2007,
    Pages 85-88,
    ISSN 1993-5269,
    rjnasci.2007.85.88,
    (https://makhillpublications.co/view-article.php?doi=rjnasci.2007.85.88)
    Abstract: The effect of using 85% buffered sucrose solution plus gelatin (&quot;S&quot;) as stabilizer and a combination of buffered sucrose/lactalbuminhydrolysate solution in ratio 20:5% (w/w), respectively with gelatin (&quot;L&quot;) as stabilizer during freeze-drying process and under varying storage temperature conditions on CBPP vaccine was investigated. It was found that both stabilizers gave good freeze-dried preparation of T<SUB>1</SUB>/44 CBPP vaccines. During the process of freeze-drying, there was a total average loss of 4.73 cfu mL <SUP>1</SUP>viable organisms in vaccine with stabilizing medium &quot;L&quot; plus gelatin and an average loss of 5.25 cfu mL <SUP>1</SUP> viable organisms for vaccine with stabilizing medium &quot;S&quot; plus gelatin. The results favoured the choice of sucrose/lactalbuminhydrolysate plus gelatin stabilizer on viability of T<SUB>1</SUB>/44 <I>Mycoplasma mycoides </I>subsp. <I>mycoides </I>during freeze-drying process. Vaccine with stabilizing medium &quot;L&quot; plus gelatin stored at <SUP>+</SUP>4°C for 15 months maintained sufficient viable mycoplasma to protect cattle. The vaccine titre dropped from 1.5×10<SUP>9</SUP>-4.3×10<SUP>7</SUP> cfu mL <SUP>1</SUP>, while vaccine with stabilizing medium &quot;S&quot; plus gelatin for the same period dropped from 3.1×10<SUP>9</SUP>-1.8×10<SUP>7</SUP> cfu mL <SUP>1</SUP>. At room temperature of 25°C, the 2 types of vaccines maintained sufficient viable mycoplasma up to 4 months, but detrimental as the titre may drop suddenly when reconstituted for vaccination in the field. Storage of this vaccine at 37°C is not recommended for the same reason stated above.
    Keywords: Stabilizer;freeze-dried;Contagious Bovine Pleuro-Pneumonia Vaccine (CBPPV);assessment of stabilizers