Elham  Raeisi, Parisa  Mousavi, Bahman  Rahimi Esboei, 
    Molecular Identification of <i>Nosema apis</i> and <i>Nosema ceranae</i> in Europian Honey Bee (<i>Apis mellifera</i>) in Northwestern of Iran,
    Research Journal of Animal Sciences,
    Volume 14,Issue 2,
    2020,
    Pages 12-15,
    ISSN 1993-5269,
    rjnasci.2020.12.15,
    (https://makhillpublications.co/view-article.php?doi=rjnasci.2020.12.15)
    Abstract: There is not enough information about the
nosomiasis infection in Iran and also in other parts of the
world. Moreover, the distinct genotype of each area in not
fully determined, so, this study was aimed to detect
microsporidians (<i>N. apis</i> and <i>N. ceranae</i>) of honeybee by
and genotyping of the parasite using PCR-RFLP
technique. During June to December, 2016, 180 adult
honeybee samples were gathered from different parts of
Northwest areas of Iran and nosomiasis was evaluated and
genotyping by microscopic and PCR-RFLP technique and
the results were confirmed by sequencing. About 75
samples out of 180 samples were microscopically positive
for nosema spores. From 75 microscopically positive
samples, 42 (56%) samples were positive by PCR
amplifications and all were diagnosed as <i>N. ceranae.</i> Our
results revealed that the <i>N. ceranae</i> opposite to the before
believes is the main strain of Nosema in Northwest of Iran
but future works with the larger sample size is
recommended.
    Keywords: Molecular identification;Nosema apis;Nosema ceranae;Europian honey bee;strain;size