Tsegu  Kiros, Negussie  Bussa, 
    Phytochemical Screening and Thin Layer Chromatographic Analysis of <i>Arachis hypogaea</i> L.
Seed Extracts,
    Plant Sciences Research,
    Volume 12,Issue 1,
    2020,
    Pages 1-6,
    ISSN 1995-476x,
    psres.2020.1.6,
    (https://makhillpublications.co/view-article.php?doi=psres.2020.1.6)
    Abstract: <i>Arachis hypogaea</i> L. belongs to the genus
<i>Arachis</i>, family Leguminoseae is a legume plant. The
seed of <i>Arachis hypogaea</i> contains various bioactive
phytochemicals in addition to the identified oil
constituents and other sources of nutrition. However,
reports regarding the role of the seed part of <i>Arachis
hypogaea</i> as source of phytochemicals and other
pharmaceuticals were very minimal. The objective of this
study was therefore to investigate the presence of various
phytochemicals and analysis thin layer chromatographic
profiles of crude seed extracts of <i>Arachis hypogaea</i>. The
ground powder of dried seed of <i>Arachis hypogaea</i> was
extracted using petroleum ether, methanol and aqueous,
separately by putting on an incubated orbital shaker
for 24 h. Each extracts were then filtered through
Whatman No. 1 filter paper, concentrated using rotary
vacuum evaporator and calculated the percentage yield.
Standard qualitative procedures were employed to identify
the major phytochemical constituents. The TLC profiles
of each crude seed extracts were observed through
UV-lamp followed by iodine vapour. Besides to this,
fluorescence analysis of all the crude extracts under
UV-lamp and ordinary light has been carried out. The
phytochemical analysis of different solvent extracts of
<i>Arachis hypogaea</i> seed studied herein revealed a strong,
moderate and weak presence of alkaloids, glycosides,
tannins, flavonoids, phenols, sterols, fats, oils, saponins
and quinines. And from the TLC profile, two spots with
Rf values 0.13 and 0.86 were observed in common in all
the solvent extracts of <i>Arachis hypogaea</i> seed. The
present study suggests that the seed of <i>Arachis hypogaea</i>
L. can be used as food supplements in everyday diet
andmay also useful in pharmaceutical industry being the
phytochemicals it contains can be used as precursors for
the isolation and characterization of pure bioactive
compounds employed as ingredients in drug formulation.
    Keywords: phytochemical analysis;thin layer chromatography;Arachis hypogaea;seed extracts;phytochemicals