Asit  B. Mandal, Raju  Mondal, Pranit  Mukherjee, Kanti  Meena, Sourav  Dutta, 
    <i>In-vitro</i> Culture Response and Regeneration of Plantlets in Mesta,
    Plant Sciences Research,
    Volume 12,Issue 3,
    2020,
    Pages 35-42,
    ISSN 1995-476x,
    psres.2020.35.42,
    (https://makhillpublications.co/view-article.php?doi=psres.2020.35.42)
    Abstract: An attempt was made to develop a robust
<i>in-vitro</i> regeneration protocol in mesta involving both the
cultivated species-H. <i>sabdariffa</i> and H. <i>cannabinus</i>. The
seed germination was found to be maximum (100%) in
var. HS 4288 and minimum (80%) in var. HS 7910 in H.
<i>sabdariffa</i> whereas in H. <i>cannabinus</i> maximum (90%)
and minimum (76%) germination were observed in vars.
MT 150 and AMC 108, respectively. Twenty three
combinations of plant growth regulators (NAA, BAP,
Kinetin, 2, 4-D, TDZ and IAA) in MS medium containing
vitamins of B<sub>5</sub> medium were assessedin governing
<i>in-vitro</i> culture response involving cotyledon and
hypocotyl explants. The best callus induction was
observed in case of cotyledon explant. The maximum
callus proliferation (97.8%) was noticed in-var. HS
4288 on MS containing 2 mg L<sup>&#150;1</sup> 2, 4-D, 0.1 mg L<sup>&#150;1</sup>
NAA and 2 mg L<sup>&#150;1</sup> Kinetin in H. <i>sabdariffa</i>. Whereas, in
H. <i>cannabinus</i> maximum callus proliferation (66.8%) was
observed in var. MT 150 on MS containing 0.8 mg L<sup>&#150;1</sup>
IAA and 0.05 mg L<sup>&#150;1</sup> TDZ. The adjuvants showed good
response in callus proliferation, however, no effect was
observed on shootlet regeneration. The shootlet
regeneration was found only in var. HS 4288 on MS
medium containing 3.0 mg L<sup>&#150;1</sup> BA with 0.2 mg L<sup>&#150;1</sup> IAA
and appreciable rooting on growth regulator free MS
medium.
    Keywords: Mesta;H. sabdariffa;H. cannabinus;In-vitro culture;plantlet regeneration