@article{MAKHILLIJTM202015319998,
    title = {The Effect of Giving Casein on TLR-4, NF-κB and TNF-α Expression Chorioamnion
Premature Rattus Norvegicus},
    journal = {International Journal of Tropical Medicine},
    volume = {15},
    number = {3},
    pages = {66-73},
    year = {2020},
    issn = {1816-3319},
    doi = {ijtmed.2020.66.73},
    url = {https://makhillpublications.co/view-article.php?issn=1816-3319&doi=ijtmed.2020.66.73},
    author = {Sumarno,Hari,Aulanni’am,Erry,Widjiati and},
    keywords = {Casein,TLR-4,NF-κB,TNFα,Rattus norvegicus},
    abstract = {During pregnancy, the fetus requires
substantial elements including protein for growth and
development. A pro-inflammatory cascade can cause
TNFα expression through NF-κB due to the presence of
casein which binds to the TLR. Knowing the effect of
case in supplementation diet on chorioamniont issue of
premature <i>Rattus norvegicus</i> pregnancies by evaluating
the expression of TLR-4, NF-&#954;B and TNF-&#945;. The study
was a real experimental laboratory with post-test only
control group design. The sample using 32 <i>Rattus
norvegicus</i> separated into two Groups (G). G1 who
received a regular diet of case in 40 g kg<sup>&#150;1</sup> BW/day (G1)
and compared with G2 which case in supplementation
diet 200 g kg<sup>&#150;1</sup> BW/day (G2), since, the first day of
pregnancy. Chorioamnion specimens taken on the 10, 12
and 14th days of gestation. Immunohistochemical
examination was performed and examined using the
Remmele Immuno Reactive Score index. The data was
analyzed statistically by SPSS25. LR-4 among G1 control
group) has an average value of 3.675&plusmn;2.001 (95% CI:
0.40-7.00) whereas the treatment Group (G2) has
3.037&plusmn;2.202 (95% CI: 0.20-7.20), there was no significant
difference between both groups. NF-&#954;B was 1.862&plusmn;1.138
(95% CI: 0.60-5.60) in G1 and was not significantly
difference (p = 0.055) against G2 (average 2.455&plusmn;0.608;
95% CI:2.00-4.20). TNF&#945; was also not significantly
different between G11.912&plusmn;0.655 (95% CI:0.00-2.80)
against G-2, 3.644&plusmn;2.795 (95% CI:1.00-11.40). The
administration of casein supplemented nutrition to
pregnant <i>Rattus norvegicus</i> was not a significant
difference in the expression of TLR-4, NF-&#954;B and TNF&#945;
in chorioamnion preterm pregnancy.}
    }