@article{MAKHILLRJPS20114327851,
    title = {Molecular Detection, Virus Isolation and Pathotyping of a Newcastle Diseaese 
  Virus Field Strain from Backyard Chickens in Qatar},
    journal = {Research Journal of Poultry Sciences},
    volume = {4},
    number = {3},
    pages = {28-32},
    year = {2011},
    issn = {1993-5285},
    doi = {rjpscience.2011.28.32},
    url = {https://makhillpublications.co/view-article.php?issn=1993-5285&doi=rjpscience.2011.28.32},
    author = {K.A.,M. and},
    keywords = {nested RT-PCR,RT-PCR,chicken,pathotyping,newcastle disease virus,Newcastle diseases,virus isolation},
    abstract = {A first trial of detection, isolation and pathotyping of a Newcastle Disease Virus (NDV) field strain was conducted in Qatar. NDV RNA was isolated, amplified and detected from oropharyngeal and cloacal swabs from clinically diseased backyard chickens and Amnioallantoic fluids (AAF) of infected chicken embryos using primary RT-PCR, nested RT-PCR and gel electrophoresing techniques. The 356 and 216 bp RNA fragments were retrieved in primary and nested RT-PCR, respectively. NDV was isolated, identified and pathotyped in commercial chicken embryos using Haemagglutination (HA), Haemagglutination Inhibition (HI) tests, Mean Death Time (MDT) and Intracerebral Pathogenicity Index (ICPI). The causative agent was found to be a highly virulent strain.}
    }