TY - JOUR T1 - Establishment and Characterization of a Chicken Oviduct Epithelial Cell Line from Hy-Line Variety Brown AU - Zhang, Qiuting AU - Wang, Chunsheng AU - Wu, Zhihao AU - Piao, Shanhua AU - Liang, Yang AU - An, Tiezhu JO - Agricultural Journal VL - 9 IS - 2 SP - 86 EP - 92 PY - 2014 DA - 2001/08/19 SN - 1816-9155 DO - aj.2014.86.92 UR - https://makhillpublications.co/view-article.php?doi=aj.2014.86.92 KW - Oviduct epithelial cells KW -obturated interior digesting KW -chicken KW -cell line KW -egg albumin AB - Chicken oviduct epithelial cells synthesize and secret most egg albumin can be a cell bioreactor or take part in preparation of intravital bioreactor, also are a good model for studying chicken oviductal gene expression regulation. Researchers established a chicken oviduct epithelial cell line named XYF-142, meanwhile, developed a new method, obturated interior digesting for efficiently procuring uncontaminated primary cells. The population doubling time of XYF-142 was 106.67 h. About 92.5% of 5th XYF-142 cells were diploid in chromosome analysis. The results of real-time PCR indicated that the expression level of oviductal epithelia-specific gene-SERPINB3 was higher in cells than in oviduct tissue and higher in 5th and 15th epithelia than in primary epithelia. In immunofluorescence cytochemistry detection for XYF-142, all cells expressed the marker protein-cell keratin 18. XYF-142 cells were competent to be transfected with vector DNA and transfection efficiency can reach 60%. Therefore, researchers concluded that researchers have successfully established a chicken oviduct epithelialcell line, provided a model for studying gene expression regulation and tissue-specific detection of chicken oviduct. ER -