TY  - JOUR
T1  - In Vitro Callus Induction from Seed, Leaf and Protocorm Explants of
<I>Paphiopedilum rothschildianum</I>
AU - Masnoddin, Makdi AU - Repin, Rimi AU - Abd Aziz, Zaleha 
JO  - Journal of Engineering and Applied Sciences
VL  - 13
IS  - 2
SP  - 367
EP  - 371
PY  - 2018
DA  - 2001/08/19
SN  - 1816-949x
DO  - jeasci.2018.367.371
UR  - https://makhillpublications.co/view-article.php?doi=jeasci.2018.367.371
KW  - Seed-derived protocorms
KW  -secondary protocorms
KW  -Protocorm Like Bodies (PLBs)
KW  -2
KW  -4-dichlorophenoacetic acid
KW  -1 mg/L 1-phenyl-3-(1-
KW  -23-Thiadiazol-5-yl)-urea (TDZ)
KW  -callus
AB  - This research was conducted to investigate the effects of different types of explants on the formation
of <I>Paphiopedilum rothschildianum</I> callus. Seed, Leaf Segments (LS), Seed-Derived Protocorms (SDP)
and Secondary Protocorms (SP) were cultured on half-strength (MS) Media Supplemented with 5 mg/L
2, 4-Dichlorophenoacetic acid (2,4-D) and 1 mg/L 1-phenyl-3-(1-23-Thiadiazol-5-yl)-urea (TDZ). Lower
percentage of seed 73.5&plusmn;14.3 and SP 65.0&plusmn;22.4% formed callus as compared to SDP 87.5&plusmn;16.9%. However, the
mass of callus induced was highest on SP as compared to SDP and seed explants. Leaf explants failed to form
callus and died after 40 days of culture. Callus started to form on seed, SDP and SP after 30 days of culture.
Further, study on SDP shows that 3 months old SDP cultured on medium with 1 mg/LTDZ and 4 mg/L 2,4-D was
the best explant for callus induction with 82.5%&plusmn;16.9 explant forming callus recorded as compared to 81.3&plusmn;12.5
and 72.0&plusmn;11.0 for 1 and 6 months old SDP, respectively. Calli produced from the present study slowly
regenerates into Protocorm Like Bodies (PLBs) and develop shoots and leaflets that further grow into plantlets
after 120 days of culture on development medium.
ER  -