Yongjun  Li, Wenting Li  , Taikun Li  , Guangjing Zhang  , Jiahui Zhang  , Dejun Ji  , 
    Construction of RNAi Expressing Vector Targeting the Goat Prion Protein Gene (<I>PRNP</I>),
    Journal of Animal and Veterinary Advances,
    Volume 11,Issue 8,
    2012,
    Pages 1208-1212,
    ISSN 1680-5593,
    javaa.2012.1208.1212,
    (https://makhillpublications.co/view-article.php?doi=javaa.2012.1208.1212)
    Abstract: To explore the effect of RNA interference on the suppression of goat prion protein gene (<I>PRNP</I>), small interfering RNA (siRNA) expressing vectors pSilencer4.1-siRNA1 and a negative control pSilencer4.1-siRNA2 and the eukaryotic expressing vector pSG5-prion expressing parts of goat prion protein gene were designed and constructed. The recombinants were introduced into Human Embryonic Kidney (HEK) 293T cells mediated by GeneTran. RT-PCR was carried out to detect the expression of prion protein gene. The results showed that RNAi effects induced by pSilencer4.1-siRNA1 could successfully deplete the mRNA expression of goat prion protein gene in 293T cells 48 h after transfection with both vectors compared to the negative control. The findings demonstrated that pSilencer4.1-siRNA1 was constructed successfully to transfer antagonistic substance against goat prion protein gene.
    Keywords: RNA interference;siRNA;prion protein gene;goat;antagonistic;protein