Changkang  Wang, Quanxi  Wang, Lili  Zeng, Huang  Lin, Hingqiang  Jia, Wenchang  Zhang, 
    Effect of Lactobacillin on the Mucosal Immune Function in Duodenum of Young Broiler,
    Journal of Animal and Veterinary Advances,
    Volume 11,Issue 20,
    2012,
    Pages 3843-3848,
    ISSN 1680-5593,
    javaa.2012.3843.3848,
    (https://makhillpublications.co/view-article.php?doi=javaa.2012.3843.3848)
    Abstract: The experiment was conducted to investigate the effects of 
  Lactobacillin in the mucosal immune function in duodenum of young broiler. A 
  total of 300, 1 day old broiler chickens were randomly divided into five groups 
  with four replicates per group and 15 chickens were in each replicate. The chickens 
  in the control group (goup I) were fed with the basal diet, those in goup II 
  were fed with basal diet supplemented with antibiotics and those in the rest 
  groups (goups III-V) were fed with basal diet supplemented with 50, 100 and 
  200 mg kg<SUP>-1</SUP> lactobacillin, respectively. The experiment was lasted 
  for 28 days. The intestinal villi height, the number of mucilage cells, intraepithelial 
  lymphocytes, mast cells and SIgA positive cells were detected by the technology 
  of histochemistry and immunohistochemistry. The results showed that the height 
  of intestinal villus in group II, IV was significantly more than group I (p&lt;0.01) 
  but there was not significantly difference between group II and IV. The results 
  also showed that there was more mucopolysaccharide positive cells and mucilage 
  cells in group II, group III and IV compared with group I (p&lt;0.05). Compared 
  with group I, the number of intraepithelial lymphocytes mast cells and SIgA 
  positive cell in group II and IV have noticeably increased (p&lt;0.05). It is 
  concluded that Lactobacillin added to broiler diet with 100 mg kg<SUP>-1</SUP> 
  can improve the mucosal immune function in duodenum of young broiler.
    Keywords: Lactobacillin;broilers;mucosal immune;mucilage;China