Huifang  Li, Wenqi  Zhu, Kuanwei  Chen, Weitao  Song, Jingting  Shu, Wei  Han, 
    Effects of the Polymorphisms of GHR Gene and IGF-1 Gene on Egg Quality in Wenchang Chicken,
    Research Journal of Poultry Sciences,
    Volume 3,Issue 2,
    2010,
    Pages 19-22,
    ISSN 1993-5285,
    rjpscience.2010.19.22,
    (https://makhillpublications.co/view-article.php?doi=rjpscience.2010.19.22)
    Abstract: Alleles of physiological candidate genes for reproductive traits, Growth Hormone Receptor (GHR) and Insulin-like Growth Factor-I (IGF-I) were assessed to determine the association with Haugh Unit (HU), egg weight EGW, Egg Shell Weight (ESW), Egg Shell Strength (ESS) and Egg Shell Thickness (EST) at 55-week age of Wenchang chicken (Chinese indigenous breed). PCR-RFLP technique was applied to analyze the polymorphisms of GHR intron 5 and IGF-1 of Wenchang chickens. The effects of GHR intron 5 and IGF-1 on the egg quality were analyzed. The results showed that the allele frequencies of C, D were 0.19, 0.81 for GHR-Intron 5 and the allele frequencies of A, B were 0.53, 0.47 for IGF-1, respectively. Three significant were observed for GHR and EST; for IGF-1 and ESW and for IGF-1 and ESS.
    Keywords: Chicken;GHR;IGF-1;egg quality;allele;reproductive traits