Research Journal of Biological Sciences
ISSN: Online 1993-6087ISSN: Print 1815-8846
Abstract
Two different clean-up methods for determination of Deoxynivalenol (DON) in cereals by liquid chromatography with DAD detection were compared. The efficiencies of two clean-up principles for rice extracts were compared: solid-phase and immunochemical extraction. There are advantages and disadvantages with both methods. In aspect of recovery, Immunoaffinity Columns (IAC) seemed to be more efficient but Solid Phase Extraction (SPE) column are more precise. Although both methods meet the requirements for reliability, for unknown matrices SPE method is somehow unreliable and has false positives. In terms of price SPE is less expensive because of self-made columns but IAC clean-up method is a specific method.
How to cite this article:
Zohreh Pouretedal and Azamosadat Meshkani . Comparing Determination of Deoxynivalenol in Cereals by HPLC Using Two Different Clean-Up Methods.
DOI: https://doi.org/10.36478/rjbsci.2008.968.972
URL: https://www.makhillpublications.co/view-article/1815-8846/rjbsci.2008.968.972