Research Journal of Medical Sciences
ISSN: Online 1993-6095ISSN: Print 1815-9346
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Isolation, Identification and Antibiotic Susceptibility Testing of Bacterial Pathogens from Blood Cultures of Neonates With Clinical Signs of Sepsis in A Tertiary Care Centre
B. Binish Joseph and Kiron Sukulal
Page: 231-235 | Received 24 Apr 2024, Published online: 13 Jun 2024
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Abstract
Neonatal mortality rate is one of the indications for measuring the health status of a nation. Neonates harbor an immature immune system, rendering them highly susceptible to infections that can cause life threatening consequences. In developed countries, Group B streptococci and coagulase negative staphylococci are the common etiological agents of neonatal sepsis. But in developing countries, the bacteriological profile varies, not only from place to place but also from time to time. Blood culture is the gold standard for the diagnosis of septicaemia and ideally should be done prior to starting antibiotics. Developed to recover microorganisms from blood, but each having its own limitations. Newer blood culture techniques using highly enriched media and a continuous monitoring system, as BacT/Alert system have improved the turnaround time for cultures to about 12 hours. They are highly sensitive, detecting organisms as low as1‐2 colony forming units per millilitre. A positive blood culture and antibiotic susceptibility profile is the best guide to effective antibiotic therapy This is a cross sectional with study sample size 142.Blood samples of neonates with sepsis for, isolation, identification and antibiotic susceptibility testing of bacterial pathogens from blood culture of neonates with clinical sign of sepsis from department of paediatrics in our hospital are included. 142 neonates with clinical signs of sepsis were enrolled in the study. 84(59.2%) were males and 58(40.8%) were females. Majority of the neonates (90, 63.4%) had birth weight greater 2.5kg, 37(26.1%) neonates had weight less than 2.5kg and 13(9.2%) <1.5kg and 2 of them <1 kg.116(81.7%) neonates presented with EOS and 26(18.3%) with LOS. 59(41.5%) neonates were born preterm. 80(56.3%) babies were born by lower segment caesarian section (LSCS), 56(39.4%) were by normal vaginal delivery and 6 of them by assisted vaginal delivery. 77(54.2%) neonates with clinical signs of sepsis had hyperbilirubinemia (NNHB)50(35.2%) neonates presented with respiratory distress. Among the 11 Gram positive organisms, all were susceptible to linezolid and vancomycin, 72.5% to amikacin,63.5% to ofloxacin, 54.5% to gentamicin, 9.09% each to ampicillin, cephalexin cloxacillin. Among the 7‐gram negative organisms, all were susceptible to meropenem and colistin,57.1% each to gentamicin, amikacin piperacillin‐tazobactam, 42.8% to ciprofloxacin28.5% to ofloxacin. There were 8 definite pathogens, 10 possible pathogens2 skin contaminants. In a reduced usage of antibiotics in the ICUs can prevent emergence of multidrug resistant bacteria. Moreover, hand hygiene forms the cornerstone in preventing the transmission of these resistant bugs.
How to cite this article:
B. Binish Joseph and Kiron Sukulal. Isolation, Identification and Antibiotic Susceptibility Testing of Bacterial Pathogens from Blood Cultures of Neonates With Clinical Signs of Sepsis in A Tertiary Care Centre.
DOI: https://doi.org/10.36478/10.36478/makrjms.2024.7.231.235
URL: https://www.makhillpublications.co/view-article/1815-9346/10.36478/makrjms.2024.7.231.235