Research Journal of Medical Sciences
ISSN: Online 1993-6095ISSN: Print 1815-9346
Abstract
Methicillin‐Resistant Staphylococcus Aureus (MRSA) is a significant public health threat, associated with high morbidity and mortality rates due to its resistance to multiple antibiotics. Mupirocin, a topical antibiotic, plays a crucial role in the decolonization of MRSA, preventing infections in clinical and community settings. This study employs both phenotypic (disk diffusion and minimum inhibitory concentration tests) and genotypic (PCR amplification and sequencing) methods to detect and characterize mupirocin resistance in MRSA isolates. We differentiate between low‐level and high‐level resistance, linked to different genetic determinants. Our findings reveal a concerning prevalence of high‐level mupirocin resistance, primarily due to the presence of the mupA gene. The implications of this resistance extend to the potential failure of decolonization efforts and control of MRSA outbreaks. This study underscores the necessity for routine surveillance of mupirocin resistance and the judicious use of this critical antibiotic to mitigate the spread of resistant MRSA strains.
How to cite this article:
T. Puneeth Kumar, K. Akila and K.S. Sudhindra. Detection and Molecular Characterization of Low Level and High‐Level Mupirocin Resistance in MRSA Isolates.
DOI: https://doi.org/10.36478/10.36478/makrjms.2024.7.85.90
URL: https://www.makhillpublications.co/view-article/1815-9346/10.36478/makrjms.2024.7.85.90