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Journal of Animal and Veterinary Advances

ISSN: Online 1993-601X
ISSN: Print 1680-5593
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Comparison Between the Effects of Valproic Acid and Trichostatin A on in vitro Development of Sheep Somatic Cell Nuclear Transfer Embryos

Ren Meng, Wureli Hazi, Chuangfu Chen, Shengwei Hu, Wei Ni, Zhirui He, Wujiafu Sai and Jixing Guo
Page: 1868-1872 | Received 21 Sep 2022, Published online: 21 Sep 2022

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https://doi.org/javaa.2012.1868.1872

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Abstract

The present study was carried out to assess and compare the effects of Valproic Acid (VPA) and Trichostatin A (TSA) on in vitro development of sheep Somatic Cell Nuclear Transfer (SCNT) embryos. The results showed that treatment of cloned sheep embryos with 4 mM VPA or 50 nM TSA for 24 h after activation could significantly improve blastocyst rate compared to the control (30.7 vs. 23.3 vs. 16.7%, respectively p<0.05). VPA treatment resulted in a significant higher blastocyst rate than that of TSA-treated group (p<0.05). Moreover, VPA treatment significantly increased (p<0.05) total cell number per blastocyst compared with the TSA treatment and control groups (78.8±9.3 vs. 69.6±9.7 vs. 64.1±8.6, respectively). Furthermore, VPA treatment increased expression of the development-related genes OCT4 and SOX2 in SCNT blastocysts. These results demonstrate that VPA may be more potent than TSA in supporting developmental competence of cloned embryos.

How to cite this article:

Ren Meng, Wureli Hazi, Chuangfu Chen, Shengwei Hu, Wei Ni, Zhirui He, Wujiafu Sai and Jixing Guo. Comparison Between the Effects of Valproic Acid and Trichostatin A on in vitro Development of Sheep Somatic Cell Nuclear Transfer Embryos.
DOI: https://doi.org/10.36478/javaa.2012.1868.1872
URL: https://www.makhillpublications.co/view-article/1680-5593/javaa.2012.1868.1872