Journal of Animal and Veterinary Advances
ISSN: Online 1993-601XISSN: Print 1680-5593
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Cloning and Expression of Transforming Growth Factor Beta 1 of Guanzhong Dairy Goat and its Effect on Proliferation of Goat Peripheral Blood Mononuclear Cells (PBMCs)
C.C. Shang, Y.Q. Zhao , D.Y. Cao , Y.L. Yao , D.K. Chen and J. Luo
Page: 1127-1134 | Received 21 Sep 2022, Published online: 21 Sep 2022
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Abstract
Transforming Growth Factor beta 1 (TGF-β1) is a pleiotropic cytokine that affects diverse biologic processes. In this study, the goat TGF-β1 gene was cloned and sequenced with length of 1173 bp. The predicted peptide of goat TGF-β1 contained 390 amino acids and 29 amino acids signal peptide. Phylogenetic analysis of different species available in GenBankTM based on the complete amino acid sequences of TGF-β1 showed that the goat was clustered with sheep sistered to bovine. The TGF-β1 mature peptide including 112 amino acids was expressed with a fusion protein of approximately 32 kDa. The goat PBMCs were proliferative stimulated by recombinant TGF-β1 but the proliferation of PBMCs stimulated by ConA was inhibited under co-stimulation of recombinant TGF-β1. These results suggest that the goat recombinant TGF-β1 would play an important role in regulating the immune responses and sustaining the balance of organism physiological state.
How to cite this article:
C.C. Shang, Y.Q. Zhao , D.Y. Cao , Y.L. Yao , D.K. Chen and J. Luo . Cloning and Expression of Transforming Growth Factor Beta 1 of Guanzhong Dairy Goat and its Effect on Proliferation of Goat Peripheral Blood Mononuclear Cells (PBMCs).
DOI: https://doi.org/10.36478/javaa.2012.1127.1134
URL: https://www.makhillpublications.co/view-article/1680-5593/javaa.2012.1127.1134