Journal of Food Technology
ISSN: Online 1993-6036ISSN: Print 1684-8462
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PCR Primers Designed from the UidA Gene Sequence for the Detection of Escherichia coli O157:H7
Moushumi Ghosh , R. Son , A. M. Sahilah , K. Sushil , W. L. Ooi , I. Salmah , S. H. Lim , Sunita Bansal and Abhijit Ganguli
Page: 59-62 | Received 21 Sep 2022, Published online: 21 Sep 2022
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Abstract
One of the uniqueness of Escherichia coli O157:H7 is it inability to ferment sorbitol in 24 h and its negative test with the MUG assay, although this organism carried the UidA gene which encodes for -glucuronidase in its chromosome. Primers were designed based on the sequence of the -glucuronidase gene and were evaluated in a polymerase chain reaction assays as a marker to detect E. coli O157:H7. Of the three pairs of primers tested, which produces the estimated product size of 352, 271 and 353 bp respectively, one primer pairs (UidA2F & UidA2R) was found to be more specific and useful as a marker in combination with a published primer for the H7 gene for the detection of Escherichia coli O157:H7.
How to cite this article:
Moushumi Ghosh , R. Son , A. M. Sahilah , K. Sushil , W. L. Ooi , I. Salmah , S. H. Lim , Sunita Bansal and Abhijit Ganguli . PCR Primers Designed from the UidA Gene Sequence for the Detection of Escherichia coli O157:H7.
DOI: https://doi.org/10.36478/jftech.2004.59.62
URL: https://www.makhillpublications.co/view-article/1684-8462/jftech.2004.59.62